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Meet Casey Bethel, Georgia's 2017 Teacher of the Year

College of Sciences basks in the reflected glow of a GIFTed science teacher

New award recognizes Wendy Kelly, Joseph Sadghi, and William Baron

Congratulations to Professors Wendy Kelly, Joseph Sadighi and William Baron as the inaugural recipients of the Vasser Woolley Award for Excellence in Instruction in the School of Chemistry and Biochemistry.

Kristy S. Syhapanha Puts a Foot in the Door of Her Dream Career

Kristy Southysa Syhapanha never thought of studying at Georgia Tech. She didn’t think she would qualify, so she didn’t bother – at first.

Missing Links Brewed in Primordial Puddles?

Researchers have achieved a significant advancement toward unlocking a longstanding evolutionary mystery -- how components of RNA and DNA formed from chemicals present on early Earth before life existed.

Welcome to Dr. Gutekunst

The School of Chemistry and Biochemistry is pleased to welcome Dr. Will R. Gutekunst, who will join the faculty on July 1, 2016 as an Assistant Professor.

Welcome to Dr. La Pierre

The School of Chemistry and Biochemistry is pleased to welcome Dr. Henry S. La Pierre, who will join the faculty on July 1, 2016 as an Assistant Professor with a courtesy appointment in the George W. Woodruff School of Mechanical Engineering and the Nuclear and Radiological Engineering and Medical Physics Program.

Seminars & Events

Meeting - Tuesday, August 16, 2016 - 11:00am - MoSE 3201A
Prof. Kenneth Brown - Georgia Institute of Technology
Colloquium - Thursday, September 1, 2016 - 4:00pm - MoSE G011
Prof. Christine Payne - Georgia Iinstitute of Technology
Colloquium - Thursday, September 8, 2016 - 4:00pm - MoSE G011
Prof. Jennifer DuBois - Montana State University
Biochemistry Division Seminar - Tuesday, September 13, 2016 - 4:00pm - MoSE 3201A

Featured Research

Article Title
Research Authors
Haopeng Xiao, George X. Tang, and Ronghu Wu.
Citation
Analytical Chemistry (2016), Vol. 88(6), 3324-3332
Miscellaneous Details
We thank Dr. Weixuan Chen for his valuable input and Johanna Smeekens for critical reading. This work is supported by the National Science Foundation.

The frequent modification of cell-surface proteins by N-linked glycans is known to be correlated with many biological processes. Aberrant glycosylation on surface proteins is associated with different cellular statuses and disease progression. However, it is extraordinarily challenging to comprehensively and site-specifically analyze glycoproteins located only on the cell surface. Currently mass spectrometry (MS)-based proteomics provides the possibility to analyze the N-glycoproteome, but effective separation and enrichment methods are required for the analysis of surface glycoproteins prior to MS measurement. The introduction of bio-orthogonal groups into proteins accelerates research in the robust visualization, identification, and quantification of proteins. Here we have comprehensively evaluated different sugar analogs in the analysis of cell-surface N-glycoproteins by combining copper-free click chemistry and MS-based proteomics. Comparison of three sugar analogs, N-azidoacetylgalactosamine (GalNAz), N-azidoacetylglucosamine (GlcNAz), and N-azidoacetylmannosamine (ManNAz), showed that metabolic labeling with GalNAz resulted in the greatest number of glycoproteins and glycosylation sites in biological duplicate experiments. GalNAz was then employed for the quantification experiment in statin-treated HepG2 liver cells, and 280 unique N-glycosylated sites were quantified from 168 surface proteins. The quantification results demonstrated that many glycosylation sites on surface proteins were down-regulated in statin-treated cells compared to untreated cells because statin prevents the synthesis of dolichol, which is essential for the formation of dolichol-linked precursor oligosaccharides. Several glycosylation sites in proteins that participate in the Alzheimer’s disease pathway were down-regulated. This method can be extensively applied for the global analysis of the cell-surface N-glycoproteome.

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