Menu
Close

Marchello Cavitt awarded the California Alliance for Graduate Education Postdoctoral (AGEP) Fellowship

Marchello Cavitt awarded the California Alliance for Graduate Education Postdoctoral (AGEP) Fellowship

Evelyn Maris and Breanne Hamlett are awarded ASEE SMART Fellowships

Evelyn Maris and Breanne Hamlett have each received the 2015 Science, Mathematics & Research for Transformation (SMART) Fellowship from the American Society for Engineering Education (ASEE). The Fellowship will support their dissertation studies and provide professional summer internships and post-doctoral work at the Defense Forensic Science Center (DFSC) in Forest Park, GA.

Prof. Rigoberto Hernandez Appointed as Phi Beta Kappa Society Visiting Scholar

Each year, top scholars in the liberal arts and sciences are selected to interact with and mentor Phi Beta Kappa students throughout the U.S.

Stefan France and Wendy Kelly Recognized for Mentorship

Two School of Chemistry and Biochemistry professors guide students and colleagues alike in science and career development.

Seth Marder Honored by the Materials Research Society

Regents’ Professor of Chemistry Seth Marder is the straightforward choice for a singular honor, for nonlinear reasons…

Reynolds group featured on ACS Headline Science

Professor John Reynolds and group publish new research discussing their “sunglasses on demand”, a polymer coating developed with the push of a button changes color! This research has been highlighted in the latest episode of ACS Headline Science.

Charlie Liotta: The Man, The Myth, The Legend

On February 26th people from across the country gathered at Georgia Tech to honor Charlie Liotta, Regents Professor Emeritus in the School of Chemistry and Biochemistry, and his 50 years of teaching and research.

Wu wins NSF CAREER Award

School of Chemistry and Biochemistry Assistant Professor Ronghu Wu has been awarded the National Science Foundation’s most prestigious award for early-career faculty.

Colorful and Dynamic Workshops

School of Chemistry and Biochemistry Professor Rigoberto Hernandez and Research Scientist Kyril Solntsev are organizing unique workshops in the mountains.

Seminars & Events

Meeting - Thursday, June 11, 2015 - 11:00am - MoSE 2100F
Meeting - Tuesday, June 16, 2015 - 11:00am - MoSE 3201A
Meeting - Thursday, July 16, 2015 - 11:00am - MoSE 2100F
Meeting - Tuesday, July 21, 2015 - 11:00am - MoSE 3201A

Featured Research

Article Title
Research Authors
Weixuan Chen, Johanna M. Smeekens, Ronghu Wu.
Citation
Molecular & Cellular Proteomics (2014), Vol. 13(6), 1563-1572
Miscellaneous Details
This work was supported by a start-up fund from Georgia Institute of Technology

Glycosylation is one of the most common and important protein modifications in biological systems. Many glycoproteins naturally occur at low abundances, which makes comprehensive analysis extremely difficult. Additionally, glycans are highly heterogeneous, which further complicates analysis in complex samples. Lectin enrichment has been commonly used, but each lectin is inherently specific to one or several carbohydrates, and thus no single or collection of lectin(s) can bind to all glycans. Here we have employed a boronic acid-based chemical method to universally enrich glycopeptides. The reaction between boronic acids and sugars has been extensively investigated, and it is well known that the interaction between boronic acid and diols is one of the strongest reversible covalent bond interactions in an aqueous environment. This strong covalent interaction provides a great opportunity to catch glycopeptides and glycoproteins by boronic acid, whereas the reversible property allows their release without side effects. More importantly, the boronic acid-diol recognition is universal, which provides great capability and potential for comprehensively mapping glycosylation sites in complex biological samples. By combining boronic acid enrichment with PNGase F treatment in heavy-oxygen water and MS, we have identified 816 N-glycosylation sites in 332 yeast proteins, among which 675 sites were well-localized with greater than 99% confidence. The results demonstrated that the boronic acid-based chemical method can effectively enrich glycopeptides for comprehensive analysis of protein glycosylation. A general trend seen within the large data set was that there were fewer glycosylation sites toward the C termini of proteins. Of the 332 glycoproteins identified in yeast, 194 were membrane proteins. Many proteins get glycosylated in the high-mannose N-glycan biosynthetic and GPI anchor biosynthetic pathways. Compared with lectin enrichment, the current method is more cost-efficient, generic, and effective. This method can be extensively applied to different complex samples for the comprehensive analysis of protein glycosylation.

Map of Georgia Tech

School of Chemistry & Biochemistry

901 Atlantic Drive Atlanta, GA 30332-0400

(404) 894-4002 (phone) | (404) 894-7452 (fax)