Lessons From Large-Scale Metabolic Phenotyping


Centre for Metabolomics Research, Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, BioSciences Building, Crown St., Liverpool, UK, L69 7ZB

Metabolomics is a growing discipline that allows the analysis of the thousands of structural different small molecules found within a biological system. These metabolites can be measured using a variety of different analytical approaches and we have developed gas chromatography mass spectrometry (GC-MS) and liquid chromatography mass spectrometry (LC-MS) for this purpose [1].  I shall provide an overview of metabolomics and lessons learnt from of our large-scale human serum metabolome project where we profiled 1200 healthy individuals [2]. Using these protocols we then went on to profile another ~1200 ageing individuals and identified key metabolic dysregulation which were drivers behind human frailty, which were validated in a further ~760 ageing individuals [3].

In parallel, we have been developing Raman and infrared microscopy in order to understand metabolic flux on a single cell level for bacterial community analysis.  Recent insights in metabolic flux analysis will be highlighted.