Regular exercise promotes whole-body health and prevents disease, but the underlying molecular mechanisms are incompletely understood. Here, the Molecular Transducers of Physical Activity Consortium – whose researchers include Regents' Professor and Vasser-Woolley Chair in Bioanalytical Chemistry Facundo Fernández – profiled the temporal transcriptome, proteome, metabolome, lipidome, phosphoproteome, acetylproteome, ubiquitylproteome, epigenome and immunome in whole blood, plasma and 18 solid tissues in male and female Rattus norvegicusover eight weeks of endurance exercise training. The data and analyses presented in the study serve as valuable resources for understanding and exploring the multi-tissue molecular effects of endurance training.